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Ships From Usa Cynomolgus Cd3e&cd3g/cd3 Epsilon&cd3 Gamma Protein, Hfc Tag Fast Dispatch [c2luRVBa]
SpecificationsSourceCynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It containsGln22-Asp117(CD3E)&Gln23-Thr113(CD3G).[Accession | Q95LI5.2(CD3E)&Q95LI7(CD3G)]Molecular Weight
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Ships From Usa Cynomolgus Cd3e&cd3g/cd3 Epsilon&cd3 Gamma Protein, Hfc Tag Fast Dispatch [c2luRVBa]
Specifications
|
Source |
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It containsGln22-Asp117(CD3E)&Gln23-Thr113(CD3G). [Accession | Q95LI5.2(CD3E)&Q95LI7(CD3G)] |
|
Molecular Weight |
The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
Activity |
ELISA Data:Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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