Last Chance Cynomolgus Ephb2 Protein, His Tag Equipment [QlN95kXZ]

Alternative Names

DRT, ERK, HEK5, TYRO5, CAPB, EK5, EPHT3, PCBC, Cek5, EphB2, EPHT3MGC87492, Nuk, Qek2, Sek3

Species

Cynomolgus

Expression Sequence

Val19-Pro542

Accession

A0A7N9CQH5

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Cynomolgus EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.