Budget-friendly Mouse Monoclonal Antibody To Fibrillarin, Cat# Mca-38f3 Smart Choice [eqK8n0Vy]

      The MCA-38F3 antibody has been widely used as a convenient marker of nucleoli in many different species including human, rat, mouse, zebrafish, C. elegans, D. melanogaster, yeast and many others. We recently mapped the epitope of MCA-38F3 to EYRAWNPFRSKLAAAILGGV, amino acids 133-152 of the human sequence, at the N-terminal of the globular domain, see here. The KD for this antibody in 4.578 X 10^-9M. While MCA-38F3 works well as a immunocytochemical marker it gives a relatively weak but still usable western blotting signal on mammalian cell extracts. We recommend other antibodies for western blotting of mammalian tissues such as our mouse monoclonal MCA-4A4, or rabbit and chicken polyclonals, RPCA-Fib and CPCA-Fib. All three were made against recombinant full length human fibrillarin.