Hot Item Drosophila Fmr1 Monoclonal Antibody [6a15] Collection [16JSyTlz]

Hot Item Drosophila Fmr1 Monoclonal Antibody [6a15] Collection [16JSyTlz] is a Mouse monoclonal antibody. Hot Item Drosophila Fmr1 Monoclonal Antibody [6a15] Collection [16JSyTlz] was generated using FMR1 as the antigen. It reacts with Drosophila/Arthropod. This antibody has been shown to work in applications such as: IP, ICC/IF, ELISA, WB. 

Target Antigen

Drosophila FMR1

Quantity

0.1ml 

Clone

6A15

Host

Mouse

Immunogen

Recombinant protein containing the amino-terminal 580 amino acids of His-FMR1 fusion protein of Drosophila melanogaster origin

Myeloma/fusion partners

SP2/0;

Species Reactivity

Fruit fly (Drosophila melanogaster)

Does not react with

Human

Purification

Protein A

Format

Purified antibody (from supernatant) containing PBS 0.1% sodium azide;

Applications

IP, ICC/IF, ELISA, WB

Dilutions

Optimal antibody dilution should be determined by titration, however as a guideline try at

IP: Use at 25 µg/mg of lysate

WB: Use at an assay dependent dilution. Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa)

References

Characterization of dFMR1, a Drosophila melanogaster homolog of the fragile X mental retardation protein. Wan L ( Molecular and Cellular Biology,Nov 2000) PUBMED 

Callan MA  et al. Fragile X Protein is required for inhibition of insulin signaling and regulates glial-dependent neuroblast reactivation in the developing brain. Brain Res 1462:151-61 (2012). ICC/IF ; Fruit fly (Drosophila melanogaster) PUBMED

Lim C  et al. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 470:399-403 (2011) PUBMED

Callan MA  et al. Fragile X protein controls neural stem cell proliferation in the Drosophila brain. Hum Mol Genet 19:3068-79 (2010). Fruit fly (Drosophila melanogaster) PUBMED

Monzo K  et al. Proteomic analysis reveals CCT is a target of Fragile X mental retardation protein regulation in Drosophila. Dev Biol 340:408-18 (2010) PUBMED

Kwak JE  et al. GLD2 poly(A) polymerase is required for long-term memory. Proc Natl Acad Sci U S A 105:14644-9 (2008). Fruit fly (Drosophila melanogaster) PUBMED

Monzo K  et al. Fragile X mental retardation protein controls trailer hitch expression and cleavage furrow formation in Drosophila embryos. Proc Natl Acad Sci U S A 103:18160-5 (2006). IP PUBMED

Research areas

Neuroscience; signal transduction;

Also known as

Drosophila FMR1 antibody;dFMR antibody;dFMR1 antibody;dFXR antibody;Drosophila fragile X mentalretardation protein antibody;FMRP antibody;Fragile X mental retardation 1 antibody;Fragile X mental retardation1 protein antibody;fragile X mental retardation protein antibody;FRAXA antibody;Protein FMR1antibody

Primary Description

Mouse Anti-Drosophila FMR1 [6A15]

Catalogue Number

IQ219

Database links

Entrez Gene: 37528 Fruit fly (Drosophila melanogaster)

SwissProt: Q9NFU0 Fruit fly (Drosophila melanogaster)

Unigene: 5279 Fruit fly (Drosophila melanogaster)

Specificity

Concentration

1mg/ml

Type

Isotype

 IgG1

Nature

Purity