Viral Deoxyribonuclease I (dnase I) , ≥ 500 Kunits/mg Now 80% Cheaper [SYeBCw8j]

High-Purity DNase I (≥ 500 Kunits/mg)

Our Deoxyribonuclease I (DNase I), with a purity of ≥ 500 Kunits/mg, is a premium enzymatic solution derived from bovine pancreas. This enzyme is meticulously purified through chromatography, ensuring the highest level of purity and efficiency. DNase I is a potent endonuclease that preferentially splits phosphodiester linkages adjacent to pyrimidine nucleotides, yielding polynucleotides with a free hydroxyl group at the 3’ position and a phosphate group at the 5’ position. This feature makes it an invaluable tool for various applications in molecular biology and genetic research.

Product Properties and Specifications

DNase I exhibits optimal activity at a pH of 7.8, making it highly effective in a wide range of biochemical reactions. It is activated by bivalent metals, such as calcium, and can be stabilized against proteolytic digestion by adding 5mM Ca²⁺. The inclusion of chelating agents like EDTA and SDS can inhibit the enzyme, allowing for precise control in experiments. Our product is supplied as a dialyzed lyophilized powder, ensuring stability and convenience for researchers. Key features include:

• High purity of ≥ 500 Kunits/mg
• Optimal pH of 7.8
• Activated by bivalent metals
• Inhibited by chelating agents

Practical Applications and Benefits

DNase I is widely used in molecular biology for its ability to degrade DNA, facilitating various experiments and applications. It is particularly beneficial in DNA cloning, Southern blotting, and DNA sequencing, where precise and controlled DNA degradation is essential. The high purity and efficiency of our DNase I make it an ideal choice for researchers looking to enhance the quality and reliability of their experiments. Whether you are a molecular biologist, geneticist, or biochemist, our DNase I is a must-have tool for your laboratory. Its versatile applications include:

• DNA cloning and sequencing
• Southern blotting
• Genetic research