Bestselling Deoxyribonuclease I (dnase I) , ≥ 2000 Kunits/mg, Rnase Free Accessory [3hZqtWXk]

Superior DNase I for Accurate DNA Digestion

Deoxyribonuclease I (DNase I) is a highly purified, bovine pancreas-derived endonuclease that is specifically designed for precise and efficient DNA digestion. This product, with a concentration of ≥ 2000 Kunits/mg, offers exceptional performance in various molecular biology applications. The DNase I is RNase-free, ensuring that no unwanted RNA degradation occurs during your experiments.

• High purity and specificity: This DNase I is purified through chromatography, guaranteeing the highest level of purity and minimizing cross-contamination with other enzymes.
• Optimized for DNA digestion: The DNase I is capable of splitting phosphodiester linkages preferentially adjacent to pyrimidine nucleotides, resulting in polynucleotides with free hydroxyl groups at the 3' position and phosphate groups at the 5' position.

Key Product Specifications and Features

Our DNase I is available in a convenient lyophilized powder form, making it easy to reconstitute and use. The optimal pH for this enzyme is 7.8, and it is activated by bivalent metals. To enhance stability against proteolytic digestion, it can be stabilized with 5mM Ca²⁺. This DNase I is highly efficient, as demonstrated by its ability to digest 1ug of lambda DNA in just 10 minutes at 37°C in a 50ul reaction.

• Stable and reliable: Store DNase I at -20°C for long-term stability and consistent performance.
• High unit concentration: Each unit of this DNase I causes an increase in absorbance at 260nm of 0.0001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.

Applications and Benefits

This DNase I is ideal for a wide range of applications, including DNA fragmentation, cloning, and sequencing. Its high purity and specificity make it a valuable tool for researchers in various fields, such as molecular biology, genetics, and biotechnology.

• Wide range of applications: Perfect for DNA fragmentation, cloning, and sequencing.
• Enhance your research: Achieve precise and efficient DNA digestion with this high-quality DNase I.