Viral 5m Betaine Solution, Pcr Certified - Us Warehouse [yE7hIFeS]

5M Betaine Solution: PCR Certified Superiority

Introducing the 5M Betaine Solution, a PCR Certified product designed to enhance your laboratory's PCR amplification process. This solution stands out due to its superior performance compared to traditional enhancers like formamide, DMSO, TMAC, BSA, and non-ionic detergents. The 5M Betaine Solution is a game-changer in the world of molecular biology, offering a range of benefits that make it an essential tool for any PCR lab.

Key Features and Specifications

The 5M Betaine Solution is formulated to bind preferentially to AT-rich sequences in the DNA major groove, stabilizing these regions and bringing the stability of AT and GC bonding closer to an equal level. This unique property results in a more consistent and reliable PCR amplification. Additionally, the solution has a sequence-independent destabilizing effect on all DNA, equalizing the Tm of AT-rich and GC-rich sequences and lowering the overall Tm. This feature not only improves the amplification of DNA but also reduces the formation of secondary structures in GC-rich regions.

• Stabilizes AT-rich DNA regions
• Equalizes Tm of AT and GC sequences

Practical Applications

The 5M Betaine Solution is an invaluable tool for PCR applications, where its ability to reduce secondary structure formation and stabilize DNA sequences is crucial. It aids the process of thermostable polymerases, reducing pauses in polymerization caused by secondary structures. This, in turn, prevents the polymerase from dissociating from the DNA strand, ensuring a more efficient and accurate amplification process. With its optimal concentration range of 0.8-1.6M, the solution is highly effective in improving PCR amplification results.

• Improves PCR amplification
• Enhances polymerase efficiency

Store the 5M Betaine Solution at -20℃ for long-term stability and optimal performance.