Essential Ptrham-6xhis-mbp Expression Vector For Improved Protein Solubility Perfect Gift [fLiljt9Z]

Protein Solubility Enhanced with pTrham-6XHis-MBP

The pTrham-6XHis-MBP expression vector is a cutting-edge solution designed to improve the solubility and proper folding of fusion proteins in E. coli. Engineered by Amid Biosciences, this vector leverages the rhamnose-inducible pTrham series of expression vectors, known for their high recombinant protein yields. It features an N-terminal 6xHis-MBP tag that not only enhances protein solubility but also promotes the yield and proper folding of fusion partners. This innovative vector is ideal for researchers seeking to optimize protein expression and purification processes.

Key Product Features

The pTrham-6XHis-MBP vector is packed with features that make it a valuable tool in the protein expression toolkit. Its rhamnose-inducible E. coli promoter ensures tight control of expression, minimizing basal expression in the absence of inducer. This allows for high expression levels while maintaining a low baseline, which is crucial for achieving active protein conformations. Additionally, the vector includes a TEV protease cleavage site, which enables the removal of the MBP tag post-production, providing flexibility in protein purification. The features of this vector are outlined below:

• High-level protein expression with a cleavable N-terminal 6xHis MBP tag
• MBP tag improves solubility and yield of fusion proteins
• L-Rhamnose-inducible promoter ensures minimal basal expression
• Directional cloning with a choice of restriction enzymes
• 6xHis affinity tag for efficient purification
• TEV protease cleavage site for MBP removal
• Ampicillin resistance

Practical Applications

The pTrham-6XHis-MBP vector is a versatile tool with numerous practical applications in research and biotechnology. Its ability to improve protein solubility and yield makes it particularly useful for producing proteins that are prone to forming inclusion bodies in E. coli. This vector is ideal for researchers working on protein purification, protein structure determination, and protein engineering. By facilitating the production of highly soluble and properly folded proteins, the pTrham-6XHis-MBP vector can significantly enhance the efficiency and success of protein expression and purification workflows.