Versatile Glycfree (λde3) Chemically Competent E. Coli Cells For Expression Of Non-glycosylated Recombinant Proteins 50% Off - Today Only [ouwxJfea]

Express Non-Glycosylated Recombinant Proteins with GlycFree (λDE3) E. coli Cells

GlycFree (λDE3) E. coli Cells are specifically engineered for the expression of non-glycosylated recombinant proteins in E. coli. These cells have been meticulously designed to overcome the limitations of traditional E. coli strains like BL21 and its derivatives, which can lead to the aberrant glycosylation of proteins. The GlycFree (λDE3) strain is defective in the synthesis of UDP-glucose, a key component in the glycosylation process. This unique feature ensures that your recombinant proteins are produced in their native conformation, preserving their biological activity.

Key Product Features

The GlycFree (λDE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, making it ideal for the controlled expression of both E. coli and T7 promoter-driven constructs. This versatile system allows for precise control over the expression of your recombinant proteins. Additionally, GlycFree (λDE3) is resistant to phage P1 infection, reducing the risk of contamination and ensuring a reliable and consistent expression process. Below are some of the key benefits and specifications of the GlycFree (λDE3) E. coli Cells:

• Transformation Efficiency: >1 x 10⁵ cfu/µg pUC19
• Resistance: Resistant to phage P1 infection

Applications and Usage

GlycFree (λDE3) E. coli Cells are invaluable for researchers seeking to express non-glycosylated recombinant proteins in their native form. This strain is particularly suitable for applications requiring high purity and functional activity of recombinant proteins, such as in drug discovery, biotechnology, and diagnostic testing. With a wide range of applications and a robust, reliable performance, GlycFree (λDE3) E. coli Cells are an essential tool for any laboratory focused on recombinant protein expression.