Easy To Use Dna Polymerase - Large Klenow Fragment Kit [nBhwC1g7]

DNA Polymerase Large (Klenow) Fragment is a truncated fragment of E. coli DNA Polymerase I that lacks the 5´→ 3´ exonuclease activity but retains its 5´→ 3´ polymerase, 3´→ 5´ exonuclease (proofreading) and strand displacement activities. 

Applications:

• Random primer labeling of DNA
• Labeling by fill-in 5'-overhangs of dsDNA
• Conversion of 5´-overhangs to create blunt-ends for cloning
• Removal of 3´-overhangs
• Second-strand synthesis of cDNA
• Site-directed DNA mutagenesis using synthetic oligonucleotides
• Dideoxy DNA sequencing of single- or double-stranded DNA templates
• Strand displacement amplification

Specifications:

Enzyme concentration: 5 Units/ul

Storage buffer: 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol

Optimal reaction temperature: 37 ^oC

Heat inactivation: 75 °C for 10 min

Storage: -20 ^oC

Shipping conditions: Shipped on dry ice

International Shipping:  Product requires shipping on dry ice. Please contact [email protected] for shipment estimates. 

Enzyme supplied with 10X Reaction Buffer (0.2 M Tris-HCl , pH 7.9, 0.5 M NaCl, 0.1 M MgCl₂ , 10 mM DTT)

Unit Definition: One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C under standard assay conditions.

This product is intended for laboratory research use only. Not for diagnostic purposes.