Overstock Deal Sos1 Protein, Human, Recombinant Pro Version [dDIaRuwi]

SOS1 Protein: Human, Recombinant

The Overstock Deal Sos1 Protein, Human, Recombinant Pro Version [dDIaRuwi] is a highly purified, recombinant protein derived from the human SOS1 gene. This protein plays a crucial role in the regulation of Ras and Rac guanine nucleotide exchange factor (GEF) activity. With its REM (Ras Exchanger Motif) and CDC25 (cell division cycle 25) domains, SOS1 effectively activates Ras proteins, promoting nucleotide exchange and facilitating cellular processes. This recombinant protein is expressed in E. coli and contains a polyhistidine tag at the C-terminus for easy purification. Key features include:

• High purity >90% by Coomassie staining
• Expressed in E. coli with a polyhistidine tag
• Applications in studying SOS1 GTP exchange activity and binding proteins

Product Properties and Specifications

The Overstock Deal Sos1 Protein, Human, Recombinant Pro Version [dDIaRuwi] is supplied in a storage buffer of 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, and 20% Glycerol. It has a molecular weight of approximately 58.3 kDa and is highly pure, exceeding 90% purity by Coomassie staining. The protein is stable and can be stored at -20°C for extended periods without loss of activity. Technical specifications include:

• Molecular weight: ~58.3 kDa
• Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, 20% Glycerol
• Concentration: 1.0 - 2.0 mg/mL by A280

Applications and Benefits

The Overstock Deal Sos1 Protein, Human, Recombinant Pro Version [dDIaRuwi] is an essential tool for researchers studying Ras and Rac signaling pathways. It is particularly useful for investigating inhibitors of SOS1 GTP exchange activity, identifying SOS1 binding proteins, and studying SOS1 GEF activity with different GTPases. This protein is also valuable for understanding the role of SOS1 in tumor cell survival, particularly in cells harboring a KRAS mutation. Applications include:

• Study inhibitors of SOS1 GTP exchange activity
• Identification of SOS1 binding proteins
• Study of SOS1 GEF activity with different GTPases

The Overstock Deal Sos1 Protein, Human, Recombinant Pro Version [dDIaRuwi] is a valuable resource for researchers in the fields of oncology, cell biology, and molecular biology, providing a reliable and efficient tool for their studies.