Your New Favorite Saposin B, Human, Recombinant Device [nK9tAy7f]

Saposin B: A Key Enzyme for Lipid Breakdown

Introducing Saposin B, a vital recombinant protein that plays a crucial role in the breakdown of cerebroside sulfates, also known as sulfatides, within lysosomes. This small, non-enzymatic glycosphingolipid activator protein is derived from human sources and produced using state-of-the-art recombinant technology. Saposin B's primary function is to extract target lipids from membranes, facilitating their conversion into soluble protein-lipid complexes that are then recognized by arylsulfatase A. This innovative protein offers a powerful tool for researchers seeking to understand and manage lysosomal storage disorders.

Product Specifications and Technical Details

Our recombinant human saposin B is meticulously produced in E. coli and presented as an N-terminal His-tag fusion. It is then purified through proprietary chromatography techniques and undergoes a site-specific proteolytic cleavage to remove the tag. Notably, this non-glycosylated recombinant saposin B retains its activation effects in functional in vitro assays. The product is available in a storage buffer consisting of 25 mM Phosphate Buffer, pH 7.2, 75 mM NaCl, and 50% Glycerol. With a concentration of 0.5 - 2.0 mg/mL by A280 (1% 3.4), this high-purity protein (≥90% purity by Coomassie staining) is ready for use in your laboratory research.

Applications and Benefits of Saposin B

Saposin B is an essential research tool for investigating lysosomal storage disorders and the breakdown of cerebroside sulfates. Its ability to form protein-lipid complexes and activate arylsulfatase A makes it invaluable for various laboratory applications. By using Saposin B, researchers can gain deeper insights into the mechanisms behind lipid metabolism and lysosomal function. This product is particularly beneficial for those working on therapies and treatments for lysosomal storage disorders, offering a valuable resource for advancing medical research and potentially improving patient outcomes.