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ProteoSure™ Recombinant Endoproteinase Lys-C: Advanced Proteomic Sequencing Tool

ProteoSure™ Recombinant Endoproteinase Lys-C is a cutting-edge enzyme designed for high-precision proteomic sequencing and mass spectrometry analysis. This recombinant endoproteinase is engineered from the lysyl endoproteinase of Achromobacter lyticus, offering superior specificity for cleaving amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. The enzyme is expressed in E.coli and purified through high-pressure liquid chromatography (HPLC), ensuring maximum purity and activity.

• High Specificity: Lys-C excels in cleaving specific amino acid residues, providing reliable results for proteomic analysis.
• High Purity: Purified through HPLC, Lys-C ensures minimal contamination, leading to accurate and reproducible results.

Key Features and Specifications

ProteoSure™ Recombinant Endoproteinase Lys-C boasts several key features and specifications that make it an ideal choice for proteomic research. With a molecular weight of 31 kDa and an enzyme commission number of 3.4.21.50, Lys-C is a highly active serine protease. The enzyme's recommended usage ratio to sample protein is 1:1000 to 1:20000 (w/w), with an optimal pH range of 7 - 9.5. The reaction should be performed at 30 - 37°C for 2 - 24 hours, ensuring efficient and effective protein digestion.

• Optimal pH and Temperature: Lys-C performs best at pH 7 - 9.5 and 30 - 37°C, allowing for precise control over the digestion process.
• Reproducibility: The consistent and reproducible results provided by Lys-C make it a reliable tool for proteomic research.

Applications and Benefits

ProteoSure™ Recombinant Endoproteinase Lys-C is a versatile tool for a wide range of proteomic applications. Its high specificity and purity make it suitable for use in various research fields, including drug discovery, protein engineering, and disease diagnosis. The compatibility of Lys-C with various agents, such as 0.2% SDS, 1.0% Triton, 6 M Urea, 0.5 M NaCl, and 20% Acetonitrile, further expands its practical applications.

• Wide Range of Applications: Lys-C is ideal for drug discovery, protein engineering, and disease diagnosis, making it a valuable tool for researchers in various fields.
• Compatibility: The compatibility of Lys-C with various agents ensures its use in various experimental conditions, enhancing its versatility.

With its exceptional features and applications, ProteoSure™ Recombinant Endoproteinase Lys-C is the perfect choice for your proteomic research needs.