In Stock 10l Media Kit: Mes-volvox Medium Bundle [RULqskr2]

Name: In Stock 10l Media Kit: Mes-volvox Medium Bundle [RULqskr2]
Brand: mappa-mercia.org
SKU: HBS12176792901
Price: 34.99 USD
Availability: InStock
Rating: 3.81 (31 reviews)

General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source.

Media kit includes all the necessary chemical components to prepare 10 Liters of MES-Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.

Looking for MES-Volvox Medium in 1-Liter and test tube volumes?

View/purchase prepared and sterile 1X MES-Volvox Medium

Medium At A Glance
Water Type	Freshwater
pH of Medium	6<pH<8
Contains	Micronutrients, NH₄, NO₃, Biotin, Vitamin B₁₂

Directions

For 10L Total Volume

Obtain and wear the appropriate PPE.
To approximately 9.7 Liters (9,700 mL) of dH₂O, add the first three solutions (Components #1-3) in order while stirring.
Bring total volume to 10 Liters (10,000 mL) with dH₂O.
Cover and autoclave or filter-sterilize if non-sterile water was used.
When cooled, aseptically add Component #4.
Store at refrigerator temperatures.

Note: When combined this gives a pH of 6.5 before autoclaving. Both the standard medium and medium made from the 10L Media Kit show a pH of 6.5 - 6.6 after autoclaving.

Components In Kit	Final Concentration
Ca(NO₃)₂•4H₂O	0.5 mM
MgSO₄•7H₂O	0.16 mM
Na₂glycerophosphate•5H₂O	0.16 mM
KCl	0.67 mM
NH₄Cl	0.5 mM
MES	10 mM
P-IV Metal Solution
Vitamin B₁₂
Biotin Vitamin Solution