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5-star Rated Erdschreiber's Medium Bundle Deal [q93b2z0P]
$18.99
$60.99
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5-star Rated Erdschreiber's Medium Bundle Deal [q93b2z0P]Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].DirectionsFor 3 L TotalTo 3 L of pasteurized filtered
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5-star Rated Erdschreiber's Medium Bundle Deal [q93b2z0P]
5-star Rated Erdschreiber's Medium Bundle Deal [q93b2z0P]
Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].
Directions
For 3 L Total
- To 3 L of pasteurized filtered seawater (30 ppt) aseptically add each of the sterile components in the order specified.
- Vigorously swirl the contents of the flask to mix thoroughly.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | Pasteurized Seawater | 3 L | ||
| 2 | P-IV Metal Solution | 36 mL/3 L | ||
| 3 | NaNO3 (autoclave before adding) (Fisher BP360-500) |
10 mL/3 L | 0.7 M | 2.3 mM |
| 4 | Na2HPO4•7H2O (autoclave before adding) (Sigma S-9390) |
10 mL/3 L | 0.02 M | 0.067 mM |
| 5 | Soilwater: GR+ Medium | 150 mL/3 L | ||
| 6 | Vitamin B12 | 3 mL/3 L |
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