Clearance Siglec H Monoclonal Antibody (ebio440c), Super Bright™ 600 - Going Fast [b50Czh7k]

SIGLEC H Monoclonal Antibody (eBio440c)

The SIGLEC H Monoclonal Antibody (eBio440c) is a powerful tool for researchers studying plasmacytoid dendritic cells (pDCs) and type I IFN-producing cells (IPCs). This antibody is designed to recognize Siglec-H, a protein found exclusively on pDCs or IPCs in naive mice. It is a vital resource for understanding the role of these cells in immune responses, particularly their quick response to viruses.

Key Features and Specifications

The eBio440c antibody offers several key features that make it an invaluable addition to any research laboratory. It is a member of the Ig superfamily, similar to CD33, and has been shown to bind sialic acid. Notably, it lacks the characteristic cytoplasmic ITIM domain, but this is overcome by its association with DAP12, enabling signal transduction. This antibody has been demonstrated to inhibit pDC function, specifically inhibiting IFNalpha secretion in response to CpG. Additionally, the antibody is highly effective for flow cytometric analysis, with a recommended dosage of less than or equal to 0.25 µg per test.

Applications and Benefits

The SIGLEC H Monoclonal Antibody (eBio440c) is versatile and suitable for various research applications. Its ability to inhibit pDC function makes it particularly useful in studies involving the immune response to CpG. It is also well-suited for flow cytometric analysis, as confirmed by testing on mouse splenocytes. The Super Bright™ 600 dye conjugation allows for high sensitivity and specificity, emitting at 600 nm when excited with the violet laser line at 405 nm. To maximize performance, we recommend using the Super Bright Complete Staining Buffer (Product # SB-4401) and following the datasheet for Super Bright Staining Buffer for detailed information. This antibody is a must-have for any researcher investigating pDCs and IPCs, offering a powerful tool for advancing the understanding of immune system function.