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cFluor® V670 anti-Mouse CD4 (RM4-5)

The cFluor® V670 anti-Mouse CD4 (RM4-5) antibody is a highly specialized tool for researchers focused on immunology and cell biology. This monoclonal antibody binds specifically to mouse CD4, a crucial 55-kDa protein that serves as a co-receptor for the T cell receptor (TCR) in the interaction with MHC class II molecules. The RM4-5 antibody is renowned for its high affinity and specificity, making it an essential component for phenotypic analysis of CD4 expression. With its cFluor® V670 conjugation, this antibody offers superior fluorescence properties, ensuring clear and reliable detection in flow cytometry experiments.

• High specificity for mouse CD4
• Excellent fluorescence properties with cFluor® V670
• Convenient for phenotypic analysis and signaling studies

Product Specifications and Technical Details

This antibody is produced through affinity chromatography, ensuring purity and consistency across batches. The RM4-5 antibody is conjugated to the vibrant cFluor® V670 fluorophore, which emits a strong and stable light at 670 nm. It is formatted as a Rat IgG2a isotype, making it suitable for a wide range of applications, including flow cytometry. Key specifications include a tested dilution of 5 µL per 1 million cells and optimal staining volume of 100 µL. This antibody is designed for research use only and is not intended for diagnostic procedures.

• Produced by affinity chromatography for purity
• Conjugated to cFluor® V670 for enhanced fluorescence
• Tested for flow cytometry with a suggested dilution of 5 µL per 1 million cells

Practical Applications and Benefits

The cFluor® V670 anti-Mouse CD4 (RM4-5) antibody is a powerful tool for a variety of research applications. Its ability to block the binding of alternative antibodies like GK1.5 makes it particularly useful for competitive binding assays. Researchers studying thymocytes, Th17, Treg cells, and dendritic cells will find this antibody invaluable for identifying and characterizing CD4-positive cells. Additionally, its compatibility with various experimental conditions ensures flexibility in experimental design. This product is a must-have for any laboratory engaged in immunological research, offering both efficiency and reliability.

• Facilitates competitive binding assays for CD4 expression
• Essential for characterizing T cell subsets and dendritic cells
• Flexible and reliable for various immunological research applications