Most Popular Mouse Interleukin-33 (il-33) Elisa Kit (top Rated) [4YuESktx]

This Most Popular Mouse Interleukin-33 (il-33) Elisa Kit (top Rated) [4YuESktx] from Innovative Research is intended for quantitative detection of mouse IL-33 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse IL-33 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-33 has been precoated onto 96-well plates. Standards(E.coli, S109-I266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-33 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-33 amount of sample captured in plate.

• Detection Target: Interleukin-33 (IL-33)
• Uniprot ID: Q8BVZ5)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information:

The capture antibody is a polyclonal antibody from goat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.; Immunogen sequence: 333