Your New Favorite Mouse Ferritin Elisa Kit Highly Recommended [9p3buAra]

Your New Favorite Mouse Ferritin Elisa Kit Highly Recommended [9p3buAra] from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Mouse Ferritin in biological samples. Strip well format. Reagents for up to 96 tests. In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Ferritin Reactivity: Mouse Range: 12.5 ng/ml - 400 ng/ml Sensitivity: 2.564 ng/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.