Premium Quality Rat Hypoxia Inducible Factor 1 Alpha Elisa Kit Series [l0U3XPE2]

Premium Quality Rat Hypoxia Inducible Factor 1 Alpha Elisa Kit Series [l0U3XPE2] from Innovative Research is intended for the quantitative determination of Rat Hypoxia Inducible Factor 1 Alpha in biofluid samples, such as tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . HIF1a present in samples reacts with an anti-Rat HIF1a capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-HIF1a detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active HIF1a in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat HIF1a. Detection Target: Rat Hypoxia Inducible Factor 1 Alpha Reactivity: Rat Range: 0.156-10ng/mL Sensitivity: 0.057ng/mL Storage Conditions: Store all components at 4┬░C This Rat HIF1a activity assay is for the quantitative determination of Hypoxia Inducible Factor 1 Alpha in samples including tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.