Experience Panserin 293s: Serum-free Medium For Hek293 Cells In Suspension Culture, W: L-glutamine (new) [XkmuUIib]

Introduction

PAN-Biotech Panserin 293S is a complete, ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in suspension culture.

Composition

Based on DMEM/F12 medium with additional oligopeptides (hydrolysates < 0.02% w/v), hormones and trace elements to support expression and cell growth. This product contains only traces of protein (< 0.002% w/v) and animal derived components (< 0.0003% w/v).

Application

Panserin 293S is a particularly enriched medium optimized for the growth of HEK293 cells in suspension culture. Due to its defined composition, the purification of final products (recombinant proteins, viruses) from the cell culture is more convenient and economic. This product can also be applied to adapt adherent HEK293 cells into suspension.

A switch from adherent serum-containing medium to Panserin 293S is often possible without adaptation. For those clones which do not tolerate a direct switch we recommend a primary culture with serum-containing medium and a stepwise reduction of serum towards a serum-free cultivation with Panserin 293S.

Documents

• Product Datasheet (PDS)
• Material Safety Data Sheet (MSDS)

Product References

• Kiefer, Anna et al. “Structure elucidation and biological activities of perylenequinones from an Alternaria species.” Mycotoxin research vol. 39,3 (2023): 303-316. doi:10.1007/s12550-023-00495-1

Related Products

• Panserin 293A: Serum-free medium for HEK293 cells in adherent culture
  
• Panexin NTS: Fully-Defined Serum Substitute for Suspension Cells

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