The Perfect Biocolor Sircol™ Soluble Collagen Assay (original Version) Set [wMZIVXBC]

The Perfect Biocolor Sircol™ Soluble Collagen Assay (original Version) Set [wMZIVXBC]

The Biocolor Sircol™ Soluble Collagen Assay is a cutting-edge quantitative dye-binding assay designed for the measurement of both acid-soluble and pepsin-soluble collagens. This assay is a valuable tool for researchers studying collagen synthesis and degradation, making it ideal for applications in cell culture, tissue analysis, and wound healing research. With its high sensitivity and specificity, the Sircol assay allows for the accurate quantification of collagen in complex samples, providing valuable insights into various biological processes.

Product Features and Specifications

The Sircol™ Soluble Collagen Assay boasts several key features that set it apart from other collagen assays. Its updated 96-well microplate format offers convenience and flexibility for high-throughput experiments. The improved dye formulation enhances collagen sensitivity and specificity, allowing for the detection of low concentrations of collagen. The assay has a wide linear range of 0 - 150 µg/ml or 0 - 500 µg/ml, depending on the setup, ensuring accurate quantification across a broad concentration range. The Sircol assay is suitable for all mammalian collagens, Types I to V, and can be used to monitor collagen production in situ or during in vitro cell culture. Additionally, it can measure in vitro extracellular matrix formation, making it a versatile tool for various research applications.

Practical Uses and Benefits

The Sircol™ Soluble Collagen Assay is widely used in research settings for various applications. It is particularly valuable for studying collagen synthesis during rapid growth and development, inflammation, wound healing, and tumor development. The assay can be used to monitor collagen production in cell culture systems, providing insights into the behavior of cells in a controlled environment. It is also suitable for analyzing conditioned media from 2D or 3D cell cultures, making it a powerful tool for studying extracellular matrix formation. The Sircol assay is a cost-effective and user-friendly alternative to conventional hydroxyproline analysis, offering researchers a reliable and efficient method for collagen quantification.