On Sale Plasmid #21 – Discontinued Take An Extra 90% Off [5jsEAFaK]

Efficient Protein Tagging with Plasmid #21

Plasmid #21 is a powerful tool for researchers seeking to tag and study target proteins. This plasmid offers the ability to add N-terminal CL7 and His8 tags to your proteins, enhancing their detection and purification in various experimental settings. With three cloning options available, you can easily integrate the plasmid into your research workflow without compromising on flexibility.

Key Features and Specifications

Plasmid #21 is designed for expression in E. coli and utilizes the T7 RNA polymerase for efficient transcription induction with IPTG. The plasmid features a strong promoter and terminator, ensuring robust protein expression. The N-terminal CL7 tag is placed upstream of protease cleavage sites, allowing for easy removal of the tag after protein purification. Below are the key features and specifications of Plasmid #21:

• Peptide Tags: N-terminal CL7 tag, His8 tag, and additional protease cleavage sites
• Cleavage Sites: Two PreScission protease (PSC) sites between CL7 and His8 tags, SUMO and Sortase A sites downstream of His8 tag
• Cloning Options: Three cloning sites (HindIII/XhoI, KpnI/XhoI, and Bsu36I/XhoI) provide flexibility for integrating the plasmid into various expression systems

Practical Applications and Benefits

Plasmid #21 is an invaluable resource for researchers in various fields, including protein expression, purification, and characterization. By providing a convenient way to tag and study proteins, this plasmid simplifies experimental workflows and enhances the accuracy of your results. The following are some practical applications and benefits of using Plasmid #21:

• Protein Expression: Efficient protein expression in E. coli with robust transcription and translation
• Protein Purification: Enhanced protein purification using His8 tag and protease cleavage sites for tag removal
• Protein Characterization: Facilitates the study of protein structure, function, and interactions through targeted tagging and purification