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Pfu Fusion DNA Polymerase: High Fidelity Enzyme

Discover the power of Pfu Fusion DNA Polymerase, a thermostable enzyme designed for unparalleled accuracy and efficiency in DNA amplification. Engineered from the heat-resistant microorganism Pyrococcus furiosus, this enzyme is fused with the small, non-specific DNA-binding protein Sso7d from Sulfolobus solfataricus. This fusion technology significantly enhances the enzyme's processivity and performance, ensuring smooth and error-free amplification.

• High Fidelity: Pfu Fusion generates PCR products with an error rate more than 30x lower than Taq DNA polymerase, perfect for applications requiring maximum accuracy.
• Enhanced Processivity: The fusion with Sso7d improves the enzyme's ability to synthesize long DNA strands, making it ideal for cloning and site-directed mutagenesis.

Product Specifications and Technical Details

This product comes with everything you need to perform PCR reactions, excluding dNTPs, primers, and DNA template. The kit includes:

• Pfu-sso7d DNA Polymerase: 1 unit/ul for optimal amplification performance.
• 5x Pfu polymerase buffer: 50 mM Tris-HCl, pH 8.5, 250 mM KCl, 7.5 mM MgCl2, 50 mM (NH4)2SO4, 0.5% Triton X-100 for stable enzyme activity.

For detailed product information, including the product manual and certificate of analysis, visit the provided links below.

Applications and Benefits

Pfu Fusion DNA Polymerase is an essential tool for researchers and laboratories requiring high-quality, error-free DNA amplification. Its numerous applications include:

• Cloning: Achieve precise cloning of genes and fragments with minimal error, ensuring successful experiments.
• Site-Directed Mutagenesis: Generate targeted mutations with high accuracy for genetic engineering studies.

Whether you're working with complex or GC-rich templates, Pfu Fusion DNA Polymerase delivers exceptional results, making it the go-to choice for researchers who demand the best in DNA amplification.