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Advanced Tyrode's Solution with HEPES: The Ultimate Research Tool

The Advanced Tyrode's Solution with HEPES is a cutting-edge research tool designed to meet the complex needs of biochemical experiments. This balanced salt solution is engineered to mimic the physiological environment, providing a stable and nutrient-rich medium for cell culture and tissue perfusion. With its enhanced buffer capacity, this solution ensures consistent pH levels, crucial for maintaining cellular homeostasis. Key features include:

• Improved Buffer Capacity: HEPES addition significantly increases the solution's buffer capacity, making it ideal for experiments requiring precise pH control.
• Comprehensive Nutrient Profile: The solution contains essential nutrients like glucose, calcium, and magnesium, supporting optimal cell growth and function.
• Wide pH Range: The solution maintains a pH range of 7.2-7.4, ideal for a variety of mammalian cell experiments.

Product Specifications and Technical Details

This Advanced Tyrode's Solution with HEPES is available in a convenient liquid format, ensuring easy handling and preparation. The solution is sterile, free from bacterial, fungal, and mycoplasma contamination, and contains no animal-origin ingredients. Key specifications include:

• Concentration: 1×
• Format: Liquid
• Bacterial Detection: Negative
• Fungal Detection: Negative
• Mycoplasmal Detection: Negative
• Endotoxin Level: ＜3 EU/mL
• Storage: 2-30℃ for 24 months with shading light
• Shipping: Room Temperature
• Expiration Date: 24 months

The solution is also free from phenol red, which is often used to indicate pH changes but can interfere with certain experiments.

Applications and Benefits in Research

The Advanced Tyrode's Solution with HEPES is an indispensable tool for researchers in various fields, including neuroscience, pharmacology, and cellular biology. Its applications are diverse:

• Isolated Muscle Experiments: The solution is particularly suitable for maintaining the normal physiological function of isolated enteromuscles in constant-muscle experiments.
• Cell Culture and Tissue Perfusion: It provides a stable environment for cell culture and tissue perfusion, supporting the survival and metabolism of cells and tissues in vitro.
• Pharmacological Studies: The solution is ideal for conducting pharmacological experiments that require precise control of pH and nutrient levels.

By offering a comprehensive and reliable solution for in vitro experiments, the Advanced Tyrode's Solution with HEPES helps researchers achieve accurate and reproducible results.