Portable Recombinant Human Chymotrypsin C Protein (his Tag) Device [uiyP9hSR]

Portable Recombinant Human Chymotrypsin C Protein (his Tag) Device [uiyP9hSR]

Our Portable Recombinant Human Chymotrypsin C Protein (his Tag) Device [uiyP9hSR] is a highly purified and bioactive protein derived from HEK293 cells. This protein is a member of the peptidase S1 family and exhibits chymotrypsin-like protease activity. With a purity of >95% as determined by reducing SDS-PAGE, this protein is ideal for research applications. Its broad substrate specificity, particularly its preference for cleaving on the carboxyl side of hydrophobic residues, makes it a valuable tool for understanding protein degradation and enzyme function.

Product Properties and Specifications

This recombinant protein is supplied as a 0.2 μm filtered solution in 20mM Tris-Hcl, 150mM NaCl, pH 7.5. It is stored at < -20°C for stability and is guaranteed to be endotoxin-free, with < 1.0 EU per μg of protein as determined by the LAL method. The protein has an observed molecular weight of 33-40 kDa, which is slightly higher than the calculated molecular weight of 29.0 kDa. It is tagged with a His tag, which facilitates purification and detection. This protein has not been validated for bio-activity but is expected to retain its chymotrypsin-like protease activity.

Practical Uses and Benefits

The Portable Recombinant Human Chymotrypsin C Protein (his Tag) Device [uiyP9hSR] is a versatile tool for researchers studying protein degradation, enzyme function, and pancreatitis. Its chymotrypsin-like activity and broad substrate specificity make it a valuable component in biochemical assays and protein analysis. This protein is also suitable for use in enzyme kinetics studies and as a positive control in Western blotting. By mitigating premature trypsinogen activation, CTRC plays a protective role in the pancreas, making it a key player in digestive zymogen activation and a potential therapeutic target for pancreatitis. Whether you are a researcher or a clinical scientist, this recombinant protein offers a reliable and efficient solution for your research needs.