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Recombinant Human CNTF Protein (N-His) (Active)

The Recombinant Human CNTF protein (N-His) (active) is a cutting-edge biotechnology product designed for research applications in neuroscience and cellular biology. This highly pure, recombinant protein is expressed in E. coli and is characterized by its active form and N-His tag for ease of purification and detection. The protein is bio-active, as demonstrated by its ability to induce proliferation in TF-1 cells with an ED₅₀ < 0.15 μg/mL, making it a valuable tool for studying neuronal and oligodendrocyte survival, neurite outgrowth, and neurotransmitter synthesis.

• High Purity: > 98% as determined by reducing SDS-PAGE.
• Endotoxin Level: < 0.01 EU per μg of the protein.

Product Properties and Specifications

The Recombinant Human CNTF protein (N-His) (active) is a recombinant protein with a molecular weight of 23.7 kDa, as calculated and observed. It is synthesized from the human sequence Met 1-Met 200 and is tagged with a C-His tag for enhanced stability and functionality. This protein is available as a lyophilized powder, making it convenient for storage and transport. The formulation includes 5% - 8% trehalose, mannitol, and 0.01% Tween 80 to ensure protein stability and reconstitutability.

• Storage: Stable for up to 12 months at -20 to -80℃. Reconstituted protein can be stored at 4-8℃ for 2-7 days.
• Shipping: Provided as a lyophilized powder shipped with ice packs to maintain product integrity.

Applications and Benefits

The Recombinant Human CNTF protein (N-His) (active) is an essential research tool for investigating various aspects of nervous system biology, particularly in the realms of neuron and oligodendrocyte survival, neurite outgrowth, and inflammation-related tissue destruction. This protein is ideal for researchers seeking to understand the molecular mechanisms underlying neurological diseases, neurodegenerative conditions, and the development of potential therapeutic interventions. The bio-active nature and high purity of this product make it a reliable choice for a wide range of applications, from basic research to drug discovery and development.

• Research Applications: Neuroscience, cellular biology, neurodegenerative disease research, and drug discovery.
• Target Audience: Academic and industrial researchers in neuroscience, cellular biology, and drug development.