Latest Model Recombinant Mouse Tnfr1/tnfrsf1a Protein (fc Tag)(active) Easy Assembly [XT9qD41G]

Latest Model Recombinant Mouse Tnfr1/tnfrsf1a Protein (fc Tag)(active) Easy Assembly [XT9qD41G]

The Latest Model Recombinant Mouse Tnfr1/tnfrsf1a Protein (fc Tag)(active) Easy Assembly [XT9qD41G] is a high-quality, active protein expression in HEK293 cells. This protein, also known as CD120a, is a key receptor for tumor necrosis factor-alpha (TNF-α) and plays a crucial role in mediating apoptosis, regulating inflammation, and activating transcription factor NF-κB. Its active form and Fc tag facilitate easy purification and downstream applications. Key features include:

• Expressed in HEK293 cells for optimal protein activity
• Contains a C-hFc tag for easy purification and detection
• Biologically active, as demonstrated by its ability to bind mouse TNFRSF1A-Fc

Product Specifications and Properties

This recombinant protein is characterized by its high purity (>90% as determined by reducing SDS-PAGE) and low endotoxin levels (<1.0 EU per μg as determined by the LAL method). It is formulated in sterile PBS buffer with 5% - 8% trehalose, mannitol, and 0.01% Tween 80 to ensure stability and solubility. Key specifications include:

• Calculated molecular weight: 47.4 kDa
• Observed molecular weight: 55 kDa
• Tag: C-hFc
• Biological activity: Immobilized mouse TNFa (80-235) at 10 μg/ml can bind mouse TNFRSF1A-Fc with an EC50 of 0.03-0.07 μg/ml

Applications and Benefits

The Recombinant Mouse TNFR1/TNFRSF1A Protein is ideal for various applications, including research on inflammation, apoptosis, and the NF-κB signaling pathway. It is particularly useful for studies involving TNF-α signaling and the role of TNFR1 in immune responses. This protein can also be employed in cell-based assays and as a research tool for understanding the molecular mechanisms behind TNF-α-induced cellular responses. Target audience includes:

• Researchers in immunology and inflammation
• Biologists and molecular biologists working on apoptosis and signaling pathways
• Drug discovery and development scientists