Name: Your New Favorite Recombinant Mouse Md1 Protein (his Tag) Holiday Cheer Edition [t79md5WH]
Brand: mappa-mercia.org
SKU: HBS530055981
Price: 72.99 USD
Availability: InStock
Rating: 4.28 (78 reviews)

Your New Favorite Recombinant Mouse Md1 Protein (his Tag) Holiday Cheer Edition [t79md5WH]

SKU #	PKSM040948
Expression Host	HEK293 Cells

Description

Synonyms	MD-1, MD1
Species	Mouse
Expression Host	HEK293 Cells
Sequence	Met 1-Ser 162
Accession	NP_034875.1
Calculated Molecular Weight	18.0 kDa
Observed Molecular Weight	24-30 kDa
Tag	C-His
Bio-activity	Not validated for activity

Properties

Purity	> 92 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.